mouseintestinalepithelialcellisolationprotoco

由 Y Ge 著作 · 2023 · 被引用 1 次 — This protocol allows for successful isolation of all expected intestinal epithelial cell types. For sample plots of flow cytometry analysis ...

2022年12月15日 — Our protocol describes in detail the required steps for the isolation and analysis of epithelial cells derived from mouse distal small intestine ...

由 G Burgin 著作 · 2019 — Using scissors, cut intestine into small pieces. Transfer pieces into a 50mL conical. If a 20mL pipette can't be used, the tissue needs to be ...

i) Mouse intestinal Crypt isolation. 1. Cut out intestine and place into ice cold HBSS w/o Ca++/Mg++. 2. Open intestine longitudinally.

2022年3月3日 — Here, we describe a magnetic cell separation protocol that employs anti-epithelial cellular adhesion molecule (EpCAM) antibodies to deplete ...

Complex cell signal transduction mechanisms regulate intestinal epithelial shape, polarity, motility, organelles, cell membrane components as well as ...

由 C Morral 著作 · 2023 · 被引用 1 次 — Procedure · Transfer the colon pieces into a 50 mL conical tube containing 15 mL of epithelial cell solution. · Incubate at 37 °C in a water bath ...

由 M Zeineldin 著作 · 2012 · 被引用 31 次 — In this protocol, epithelial cell adhesions are weekend by chelating Ca +2 ions followed by mechanical separation of the cells by vortexing.

Use pointed forceps to remove any membrane, blood vessels, and fat from the exterior of the intestine (Figure 1). Place the intestinal segment into a 10 cm dish ...

Find a series of step-by-step procedures and videos for the isolation, culture, passaging, and cryopreservation of mouse small intestinal and colonic crypts ...